Journal: Nature Communications

Article Title: Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice

doi: 10.1038/s41467-025-62071-8

Figure Lengend Snippet: a UMAP visualization of single-cell transcriptomes from kidneys of Gsdme -KO mice and their WT littermates. Colors represent distinct cell clusters; each dot corresponds to a single cell. b UMAP plot displaying the subclusters of PTCs. c Bubble plot showing the gene expression profiles of Gsdme across different renal cell populations. PTCs Proximal Tubular Cells, MPC Mono-nuclear Phagocyte, T cells T lymphocytes, Neutro Neutrophil, B cells B lymphocytes, CD Collecting Duct, LOH Loop of Henle, ECs Endothelial Cells, DCT Distal Convoluted tubule, NK cells Natural Killer cells, Myofib Myofibroblasts, PECs/Podo Parietal Epithelial Cells/Podocyte. d Bubble plot showing the gene expression profiles of Gsdme across different WT PTCs subpopulations. e Proportions of different PTCs subpopulations in different groups. f Pseudotime analysis illustrating the differentiation trajectory of PTCs. g Visualization of PTCs differentiation trajectories stratified by the indicated experimental condition. h Heatmap showing differentially expressed genes (DEGs, rows) along pseudotime (columns), hierarchically clustered into four distinct expression patterns, each corresponding to a specific trajectory phase. Right panel: GO-BP terms associated with each gene expression pattern.

Article Snippet: The immortalized human kidney tubular epithelial cell line (HK-2, Cat: GF1117C) and the human embryonic kidney 293 T cell line (HEK-293T, Cat: GF3087C) were purchased from American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS; Bio-Channel).

Techniques: Gene Expression, Expressing